Im struggling with how to set the spatial frequency parameters. According to Church’s criteria, I would want the spatial resolution of the instrument to be 0.8um and the trace length to
be 3 mm but I’m not sure how to go about this. Can anybody help?
What instrument are you using, is it one of our Profilm3D systems?
Is there a specific image you’re having an issue with?
You cannot set the cutoff length to a larger value than the image dimensions, so, if for example you acquired the data with a 10x objective on the Profilm3D system, your field of view is ~2.5x2.5mm, and you would need to do image stitching before using a 3mm cutoff length.
Feel free to DM me with more details.